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KMID : 0545120110210121228
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 12 p.1228 ~ p.1235
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Identification of Virulence Factors in Vibrio vulnificus by Comparative Transcriptomic Analyses Between Clinical and Environmental Isolates Using cDNA Microarray
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Kim In-Hwang
Kim Byung-Soo
Lee Kyung-Shin
Kim Ik-Joong
Son Jee-Soo
Kim Kun-Soo
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Abstract
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We compared the gene expression among four clinical and five environmental V. vulnificus isolates, using a cDNA microarray containing 131 genes possibly associated with pathogenicity, transport, signal transduction, and gene regulations in the pathogen. cDNAs from total RNAs of these isolates were hybridized into the cDNA microarray using the cDNA of the wild-type strain MO6/24-O as a reference. We focused on selecting differentially expressed (DE) genes between clinical and environmental isolates using a modified t-statistic. We could detect two statistically significant DE genes between virulent isolates and lessvirulent isolates with a marginal statistical significance (pvalue of 0.008). These were genes putatively encoding pilin and adenlyate cylase. Real time-PCR confirmed that these two selected genes transcribed in significantly higher levels in virulent isolates than in less-virulent isolates. Mutants with lesions in the gene encoding pilin showed significantly higher LD50 values than that of wild type.
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KEYWORD
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Vibrio vulnificus, Virulence factors, cDNA microarray, pilin
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